Composite

Part:BBa_K197032:Design

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)

{Pbad.rbs.prepro.StrepTag}{type IIIs Needle Complex scFV}{upaG_short}(dblterm)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
    Illegal AgeI site found at 2383
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

BglBrick assembly

References